Journal: Scientific Reports

Article Title: Microbial stress mediated intercellular nanotubes in an anaerobic microbial consortium digesting cellulose

doi: 10.1038/s41598-017-18198-w

Figure Lengend Snippet: Elemental mapping of the microbial cells and appendages. The following images represent the elemental mapping that was performed using an Energy Dispersive X-ray spectrometer (EDX) (Oxford Instrument, X-max, 80 mm 2 ) attached to the FE-SEM to determine elemental compositions of the microbial cells as well as extracellular appendages. Pointers indicate microbial cells (yellow), iron oxide nanoparticles (red) and intercellular appendages (green). ( a ) Elemental mapping of the image and the representative elements (C, N, O, Na, S, Cl, K, Ca and Fe) detected in day 12 test reactor sample. Scale bar represents 2 µm. ( b , c ) In this day 12 control reactor sample, the individual points at which EDX spectra were obtained has been highlighted and the respective elemental compositions are shown in the adjacent graphs. The results indicate that the appendages are cell derived and have similar elemental compositions. Scale bar represents 9 µm.

Article Snippet: Elemental mapping was performed using an Energy Dispersive X - ray spectrometer (EDX) (Oxford Instrument, X-max, 80 mm 2 ) attached to the FE-SEM.

Techniques: Derivative Assay